Isolation and Molecular characterization of Marek’s disease virus from layer chickens in Egypt

نویسندگان

چکیده

Marek’s disease virus is a lymphomatous neuropathic of domestic fowl. In Egypt, poultry flocks are suffering from recurrent uprising MD outbreaks due to increased virulence the virus. this study, total 10 clinical samples were collected 6-8 months old vaccinated layer chickens depression, ataxia and paralysis. These examined for presence MDV by PCR using specific primers ICP4 gene. was detected in 5 out samples. One positive isolated partially sequenced gene (F1352-S4).  The results proved that it genetically characterized as very virulent strain Marek's type 1 resembling Gallid herpesvirus 2 isolates Europe, China, Africa India. There characteristic serine residue at coding region encoding reverse complement protein found only Egyptian (F1352-S4) EU-1 virus, IND-KA-02-06, JL/1404 Debre-zeit/01/2013 tracing original CV1988. obtained indicated importance continuous monitoring infection chicken flocks.

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ژورنال

عنوان ژورنال: Journal of Veterinary Medical Research

سال: 2021

ISSN: ['2357-0520', '2357-0512']

DOI: https://doi.org/10.21608/jvmr.2021.59613.1032